| Autor: |
Gromova EV, Poliakov NB, Grinkevich VA |
| Jazyk: |
ruština |
| Zdroj: |
Bioorganicheskaia khimiia [Bioorg Khim] 2012 Jan-Feb; Vol. 38 (1), pp. 18-30. |
| DOI: |
10.1134/s1068162012010104 |
| Abstrakt: |
We have conducted a research of mitochindrial internal membrane proteins. This fraction has been received in the form of submitochondrial particles (SMP). SMP have been processed by trypsinum, and the received peptides have been separated from so-called "smoothfaced vesicles". "Smoothfaced vesicles" were blasted, proteinse and peptides were processed by cyanogen bromide and trypsinum. We have received two groups of tryptic peptides and analyzed them separately with the help of proteomic methods, such as chromatography, mass spectrometry and protein identification in different databases. To identify more proteins and find minor components of mitochindrial proteome we have considered possible non-specific fragmentation of proteins. 298 proteins have been identified, we also have conducted the analisys of their functions and cell localization. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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