[The in-vitro effects of arbidol hydrochloride against 2009 new influenza virus A (H1N1)].

Autor:	Zhang XQ; University of California, San Diego, CA 92093, USA., Dai JP, Cai BQ
Jazyk:	čínština
Zdroj:	Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae [Zhongguo Yi Xue Ke Xue Yuan Xue Bao] 2012 Apr; Vol. 34 (2), pp. 126-9.
DOI:	10.3881/j.issn.1000-503X.2012.02.005
Abstrakt:	Objective: To detect the in-vitro effects of arbidol hydrochloride against 2009 new influenza virus A (H1N1). Methods: The activity of arbidol hydrochloride against 2009 new influenza virus A (H1N1) was determined in MDCK cell cultures. Hemagglutination assay, observation of cytopathic effects, RT-PCR and quantitative RT-PCR tests were performed for determination of virus titers. Inhibition concentration 50% and cytotoxic concentration 50% were calculated with Chou's Menu of Dose-Effect Program. Results: Arbidol hydrochloride showed low cytotoxicity (cytotoxic concentration 50%>100 μmol/L)and significant anti-2009 new influenza virus A (H1N1) activity in cell cultures. Inhibition concentration 50% were (5.5 ± 0.9), (3.4 ± 0.8), and (1.5 ± 0.2) μmol/L in hemagglutination assay, cytopathic effect test, and quantitative RT-PCR assay, respectively. Conclusion: Arbidol has low cytotoxicity and high anti-virus activity and can effectively trigger the activities of interferon and immune response, and therefore can be a valuable anti-influenza virus drug.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu