Functional expression of the multi-subunit type calcium/proton antiporter from Thermomicrobium roseum.
| Autor: | Morino M; Graduate School of Life Sciences, Toyo University, Oura-gun, Gunma, Japan., Ito M |
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| Jazyk: | angličtina |
| Zdroj: | FEMS microbiology letters [FEMS Microbiol Lett] 2012 Oct; Vol. 335 (1), pp. 26-30. Date of Electronic Publication: 2012 Jul 30. |
| DOI: | 10.1111/j.1574-6968.2012.02634.x |
| Abstrakt: | Multiple resistance and pH adaptation (Mrp) antiporters are widely distributed in various prokaryotes and have been reported to function as a hetero-oligomeric monovalent cation/proton antiporter, which exchanges a cytoplasmic monovalent cation (Na(+), Li(+), and/or K(+)) with extracellular H(+). In many organisms, they are essential for survival in alkaline or saline environments. Here, we report that the Mrp antiporter from the thermophilic gram-negative bacterium, Thermomicrobium roseum, does not catalyze monovalent cation/proton antiport like the Mrp antiporters studied to date, but catalyzes Ca(2+)/H(+) antiport in Escherichia coli membrane vesicles. (© 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.) |
| Databáze: | MEDLINE |
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