| Autor: |
Leigh SY; Stony Brook University (SUNY), Department of Biomedical Engineering, Stony Brook, NY 11794, USA., Liu JT |
| Jazyk: |
angličtina |
| Zdroj: |
Optics letters [Opt Lett] 2012 Jun 15; Vol. 37 (12), pp. 2430-2. |
| DOI: |
10.1364/OL.37.002430 |
| Abstrakt: |
We present a miniature microelectromechanical systems-based dual-axis confocal microscope capable of spatially coregistered fluorescence and reflectance imaging at multiple wavelengths. This device has a 10 mm diameter scan head with a 2 mm diameter tip for convenient use during surgery to guide tumor resection. The microscope has an adjustable focal depth of 20-200 micrometers and is capable of imaging with an axial resolution of 9 micrometers and in-plane resolution of 4 micrometers over a field of view of 450×450 micrometers. Simultaneous two-color imaging of individual optical sections is achieved by using a pair of grating-prism assemblies to compensate for chromatic dispersion in the 2 mm diameter gradient index relay lens at the distal tip of the device. Experimental measurements of the axial response of the microscope, as well as two-color images of a reflective bar target and fresh mouse brain tissues, demonstrate the performance of our device and its potential for multicolor in vivo optical sectioning microscopy. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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