| Autor: |
Mehta P; Department of Biochemistry and Cell Biology, Stony Brook University, Centers for Molecular Medicine, Stony Brook, NY, USA., Woo P, Venkataraman K, Karzai AW |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2012; Vol. 905, pp. 273-89. |
| DOI: |
10.1007/978-1-61779-949-5_18 |
| Abstrakt: |
Ribosomes are large complexes of RNA and protein that perform the essential task of protein synthesis in the cell. Ribosomes also serve as the initiation point for several translation-associated functions. To perform these tasks efficiently, ribosomes interact with a myriad of nonribosomal proteins and RNAs. Given that most of these interactions are transient, purification of the interacting factors in complex with the ribosome can be a challenging undertaking. Here, we review methods commonly used to isolate ribosomes and study ribosome-associated factors. We also discuss crucial parameters for designing and executing ribosome association studies. Finally, we present a detailed protocol for reporter based enrichment assays that are employed to selectively isolate ribosomes translating a particular message of interest. These protocols can be used to study a wide range of ribosome-associated functions. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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