| Autor: |
Lowe AJ; Graduate Field of Microbiology, Cornell University, Ithaca, NY, USA. alowe@srcinc.com, Bardliving CL, Batt CA |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2012; Vol. 899, pp. 265-75. |
| DOI: |
10.1007/978-1-61779-921-1_17 |
| Abstrakt: |
Endotoxin removal is critical when producing therapeutic proteins in bacterial systems. This hydrophobic compound can be removed through chromatography or filtration, but presents unique challenges dependent upon protein composition as well as production scale. Here we present a robust method for endotoxin removal at the pilot production scale using fast protein liquid chromatography and buffers specifically engineered for endotoxin removal. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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