| Autor: |
Grieco SH; Centre for Blood Research, University of British Columbia, Vancouver, BC V6T 1Z3, Canada. sunghye.grieco@ubc.ca, Wong AY, Dunbar WS, MacGillivray RT, Curtis SB |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of industrial microbiology & biotechnology [J Ind Microbiol Biotechnol] 2012 Oct; Vol. 39 (10), pp. 1515-22. Date of Electronic Publication: 2012 Jun 20. |
| DOI: |
10.1007/s10295-012-1148-3 |
| Abstrakt: |
Previously, we used computer-controlled fermentation technology to improve the yield of filamentous phage produced in Escherichia coli by 10-fold (Grieco et al., Bioprocess Biosyst Eng 32:773-779, 2009). In the current study, three major fermentation parameters (temperature, dissolved oxygen [DO], and pH) were investigated using design of experiments (DOE) methodology. Response surface methodology (RSM) was employed to create a process model and determine the optimal conditions for maximal phage production. The experimental data fitted best to a quadratic model (p < 0.0001). Temperature and pH, but not DO, proved to be significant variables. The model predicted a theoretical optimal condition for maximal bacteriophage production at temperature of 28.1 °C and pH 6.9. A validation run resulted in phage production [3.49 × 10(11) transducing units (TU)/mL] comparable to the predicted value (2.86 × 10(11) TU/mL). This represented a 7-fold increase in phage production above that obtained without optimization, resulting in a 70-fold increase above that achieved by shake flask culture alone. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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