Development of an amperometric immunosensor for detection of staphylococcal enterotoxin type A in cheese.

Autor: Pimenta-Martins MG; Department of Chemistry, State University of Ceará, Av. Paranjana, 1700, Fortaleza-CE, Brazil., Furtado RF, Heneine LG, Dias RS, Borges Mde F, Alves CR
Jazyk: angličtina
Zdroj: Journal of microbiological methods [J Microbiol Methods] 2012 Oct; Vol. 91 (1), pp. 138-43. Date of Electronic Publication: 2012 Jun 16.
DOI: 10.1016/j.mimet.2012.05.016
Abstrakt: This paper reports a novel electrochemical immunosensor for the sensitive detection of staphylococcal enterotoxin A (SEA) based on self-assembly monolayer (SAM) and protein A immobilization on gold electrode. Three different methods of protein A immobilization were tested: physical adsorption, cross-linking using glutaraldehyde and covalent binding after activation with N-hydroxysuccinimide (NHS)/N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) on cysteamine-modified gold electrode. The EDC/NHS method for protein A immobilization was selected to lead development of the biosensor. The coating steps of the surface modification were characterized by cyclic voltammetry and the biosensor response by chronoamperometry. The advantages of the immunosensor were exposed in its high sensitivity and specificity. The proposed amperometric immunosensor was successfully used for determination of SEA in contaminated and non-contaminated cheese samples with excellent responses.
(Copyright © 2012 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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