Periodontal regeneration employing gingival margin-derived stem/progenitor cells: an animal study.

Autor: Fawzy El-Sayed KM; Clinic for Conservative Dentistry and Periodontology, School of Dental Medicine, Christian Albrechts University, Kiel, Germany., Paris S, Becker ST, Neuschl M, De Buhr W, Sälzer S, Wulff A, Elrefai M, Darhous MS, El-Masry M, Wiltfang J, Dörfer CE
Jazyk: angličtina
Zdroj: Journal of clinical periodontology [J Clin Periodontol] 2012 Sep; Vol. 39 (9), pp. 861-70. Date of Electronic Publication: 2012 Jun 13.
DOI: 10.1111/j.1600-051X.2012.01904.x
Abstrakt: Aim: This study investigated the periodontal regenerative potential of gingival margin-derived multipotent postnatal stem/progenitor cells.
Material and Methods: Periodontal defects were induced at six sites in eight miniature pigs in the premolar/molar area (-4 weeks). Autologous cells isolated from the gingival margin were magnetically sorted using STRO-1 antibodies and characterized flow cytometrically for the expression of CD14, CD31, CD34, CD45, CD117 and STRO-1 surface markers. Colony formation and multilineage differentiation potential were tested. The cells were expanded and loaded on deproteinized bovine cancellous bone (DBCB) and Collagen scaffolds. Within every miniature pig, six periodontal defects were randomly treated with loaded-DBCB (test group 1), unloaded-DBCB (control group 1), loaded-Collagen scaffolds (test group 2), unloaded-Collagen scaffolds (control group 1), scaling and root planing (negative control 1) or left untreated (negative control 2). Differences in clinical attachment level (ΔCAL), probing depth (ΔPD), gingival recession (ΔGR) and radiographic defect volume (ΔRDV) between baseline and 12 weeks, as well as histological attachment level (HAL), junctional epithelium length (JE) and connective tissue adhesion (CTA) after 12 weeks were evaluated.
Results: Isolated cells showed stem/progenitor cell characteristics. Cell-loaded scaffolds showed higher ΔCAL, ΔPD, ΔGR, HAL and lower JE and CTA compared with unloaded scaffolds and negative controls. The sort of scaffold had no significant influence on the measured outcomes.
Conclusion: Gingival margin-derived stem/progenitor cells show significant periodontal regenerative potential.
(© 2012 John Wiley & Sons A/S.)
Databáze: MEDLINE
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