| Autor: |
Grinev VV, Severin IN, Posrednik DV, Kosmacheva SM, Potapnev MP |
| Jazyk: |
ruština |
| Zdroj: |
Genetika [Genetika] 2012 Mar; Vol. 48 (3), pp. 389-400. |
| Abstrakt: |
The efficiency of human bone marrow (BM) mesenchymal stem cell (MSC) transduction with a bicistronic lentivirus vector was estimated, and the stability of transgene expression in genetically modified MSCs was determined. First-passage BM MSCs were capable of efficient transduction with the bicistronic lentivirus vector. The transduction efficiency depended on the multiplicity of infection (MOI), being 64 +/- 6.5 and 88.6 +/- 2.9% at MOI 10 and 20, respectively. The lentivirus transduction efficiency proved independent on the number of passages of a BM MSC culture, and expression of the egfp and dsRed1 transgenes in genetically modified MSCs remained stable for one month of culturing. A comparison showed that the level of egfp and dsRed1 transgene expression was preserved upon hepatogenic differentiation in vitro. The results provide a basis for further development of multigenic modification of human BM MSCs for research and/or therapeutic purposes. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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