| Autor: |
Kim HJ; Foreign Animal Disease Division, Animal Plant and Fisheries Quarantine and Inspection Agency, Anyang, Gyeonggi-do, Korea., Tark DS, Lee YH, Kim MJ, Lee WY, Cho IS, Sohn HJ, Yokoyama T |
| Jazyk: |
angličtina |
| Zdroj: |
The Journal of veterinary medical science [J Vet Med Sci] 2012 Oct; Vol. 74 (10), pp. 1377-80. Date of Electronic Publication: 2012 Jun 05. |
| DOI: |
10.1292/jvms.12-0061 |
| Abstrakt: |
Elk prion protein (PrP(C)) has been confirmed to be capable of rendering rabbit epithelial RK13 cells permissive to temporal infection by chronic wasting disease (CWD) prions. The present study satisfactorily generated persistently CWD prion-affected RK13 cells (RKC1-11) using elk PrP(C) expressing cells (elkRK13) that were generated via the lentiviral expression system with high efficiency. The elkRK13 cells have been shown to be permissive to accumulation of abnormal isoforms of prion protein (PrP(Sc)) resulting from CWD prions up to 97 serial passages thus far. This novel prion-affected cell line will help facilitate investigation of the molecular basis of CWD prion pathogenesis and confirmation of CWD prion infectivity in vitro. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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