| Autor: |
Shaluei F; Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, P.O. Box 45165-386, Gorgan, Iran. fardin.shaluei@gmail.com., Hedayati A; Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, P.O. Box 45165-386, Gorgan, Iran., Jahanbakhshi A; Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, P.O. Box 45165-386, Gorgan, Iran., Baghfalaki M; Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, P.O. Box 45165-386, Gorgan, Iran. |
| Jazyk: |
angličtina |
| Zdroj: |
Fish physiology and biochemistry [Fish Physiol Biochem] 2012 Dec; Vol. 38 (6), pp. 1627-1634. Date of Electronic Publication: 2012 Jun 03. |
| DOI: |
10.1007/s10695-012-9659-4 |
| Abstrakt: |
The objective of the study was to evaluate the efficacy of 2-phenoxyethanol (2-PE) as an anesthetic in great sturgeon under two experiments. First, fish were exposed to 0.1, 0.3, 0.5, 0.7 and 0.9 mL/L 2-PE, and time to induction (deep anesthesia) and recovery from anesthesia were measured. At concentration of 0.1 mL/L, 2-PE failed to induce deep anesthesia in fish, whereas at concentrations of 0.7 and 0.9 mL/L, all the fish were anaesthetized within 3 min of exposure. For assessing the impact of effective concentrations of 2-PE on physiological responses of great sturgeon, hematological indices, plasma metabolites, electrolytes, enzymes and cortisol levels were measured. The use of 2-PE induces a significant increase in RBC values at 0.3 mL/L concentration and a parallel increase in hemoglobin and hematocrit values. 2-PE anesthesia had no effect on WBC, MCV, MCH and MCHC levels when compared to control group. Serum glucose, cholesterol and cortisol levels were significantly high in 0.3 and 0.5 mL/L 2-PE. Moreover, AST levels were increased in fish exposed to the 0.3 mL/L 2-PE comparing with the control group (P < 0.05). There were no significant differences in serum levels of total protein, triglycerides, ALP, ALT, Cl(-), Na(+), K(+), Ca(2+) and Mg(2+). In this study, alteration in hematological and serum biochemical indices was time-dependent. This study demonstrates that rapid induction of deep anesthesia with a relatively high concentration of 2-PE (0.7 and 0.9 mL/L) was associated with the lowest effects on the hematological and serum biochemical indices in great sturgeon and therefore would be recommended as eligible doses for hematological studies in this species. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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