| Autor: |
Andreeva IP, Vorob'eva NT, Vinnitskiĭ LI, Bogush SS, Gavrilova EM, Egorov AM |
| Jazyk: |
ruština |
| Zdroj: |
Biomeditsinskaia khimiia [Biomed Khim] 2011 Sep-Oct; Vol. 57 (5), pp. 554-61. |
| DOI: |
10.18097/pbmc20115705554 |
| Abstrakt: |
A test-system based on enzyme-linked immunosorbent assay (ELISA) for quantitative determination of cyclosporin A (CSA) in human whole blood has been developed. The detection limit of the method was 25 ng/ml, the linearity of the method in the concentration range 60-1400 ng/ml varied from 94 to 105%, the variation coefficient did not exceed 8%. The novel method exhibited good correlation with radio-immunoassay and polarization fluoroimmunoassay methods; the linear regression coefficients were 0.965 and 0.983, respectively. The developed test system is stable for at least 9 months when stored at 4 degrees C and can be used in clinical practice. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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