| Autor: |
Heinze RJ; Institute for Biochemistry, FB 08, Justus Liebig University, Heinrich-Buff-Ring 58, D-35392 Giessen, Germany., Sekerina S, Winkler I, Biertümpfel C, Oretskaya TS, Kubareva E, Friedhoff P |
| Jazyk: |
angličtina |
| Zdroj: |
Molecular bioSystems [Mol Biosyst] 2012 Jul 06; Vol. 8 (7), pp. 1861-4. Date of Electronic Publication: 2012 May 24. |
| DOI: |
10.1039/c2mb25086a |
| Abstrakt: |
The DNA repair protein MutS forms clamp-like structures on DNA that search for and recognize base mismatches leading to ATP-transformed signaling clamps. In this study, the mobile MutS clamps were trapped on DNA in a functional state using single-cysteine variants of MutS and thiol-modified homoduplex or heteroduplex DNA. This approach allows stabilization of various transient MutS-DNA complexes and will enable their structural and functional analysis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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