Specific detection of all members of the Venezuelan Equine Encephalitis complex: development of a RT-Nested PCR.
| Autor: | Pisano MB; Instituto de Virología Dr. J. M. Vanella, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina. pisanomb@yahoo.com, Seco MP, Ré VE, Farías AA, Contigiani MS, Tenorio A |
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| Jazyk: | angličtina |
| Zdroj: | Journal of virological methods [J Virol Methods] 2012 Dec; Vol. 186 (1-2), pp. 203-6. Date of Electronic Publication: 2012 May 18. |
| DOI: | 10.1016/j.jviromet.2012.05.009 |
| Abstrakt: | Venezuelan Equine Encephalitis (VEE) complex belongs to alphavirus genus in the family Togaviridae. Several species of this complex are pathogenic to humans. VEE infections can produce severe or mild disease, and many cases remain undiagnosed. A specific and sensitive reverse transcriptase nested polymerase chain reaction (RT-Nested PCR) method was developed for the detection of all VEE subtypes, including Rio Negro Virus (RNV) (subtype VI), which circulates only in Argentina. Degenerated primers were designed and thermal cycling parameters were standardized. This technique is suitable for rapid and specific detection of these viruses, and may be useful for diagnosis and surveillance. (Copyright © 2012 Elsevier B.V. All rights reserved.) |
| Databáze: | MEDLINE |
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