Comparison of two eukaryotic systems for the expression of VP6 protein of rotavirus specie A: transient gene expression in HEK293-T cells and insect cell-baculovirus system.

Autor: da Silva Junior HC; Laboratório de Virologia Comparada e Ambiental, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz. Av. Brasil, 4365, Pavilhão Hélio & Peggy Pereira, Rio de Janeiro, RJ 21040-360, Brazil. haroldo.cid@bio.fiocruz.br, da Silva E Mouta Junior S, de Mendonça MC, de Souza Pereira MC, da Rocha Nogueira A, de Azevedo ML, Leite JP, de Moraes MT
Jazyk: angličtina
Zdroj: Biotechnology letters [Biotechnol Lett] 2012 Sep; Vol. 34 (9), pp. 1623-7. Date of Electronic Publication: 2012 May 11.
DOI: 10.1007/s10529-012-0946-z
Abstrakt: The VP6 protein of rotavirus A (RVA) is a target antigen used for diagnostic assays and also for the development of new RVA vaccines. We have compared the expression of VP6 protein in human embryonic kidney (HEK293-T) cells with results obtained using a well-established insect cell-baculovirus system. The recombinant VP6 (rVP6) expressed in HEK293-T cells did not present degradation and also retained the ability to form trimers. In the insect cell-baculovirus system, rVP6 was expressed at higher levels and with protein degradation as well as partial loss of ability to form trimers was observed. Therefore, HEK293-T cells represent a less laborious alternative system than insect cells for expression of rVP6 from human RVA.
Databáze: MEDLINE
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