| Autor: |
Wanji S; Research Foundation for Tropical Diseases and the Environment, Buea, South West Region, Cameroon. swanji@yahoo.fr, Kengne-Ouafo AJ, Eyong EE, Kimbi HK, Tendongfor N, Ndamukong-Nyanga JL, Nana-Djeunga HC, Bourguinat C, Sofeu-Feugaing DD, Charvet CL |
| Jazyk: |
angličtina |
| Zdroj: |
The American journal of tropical medicine and hygiene [Am J Trop Med Hyg] 2012 May; Vol. 86 (5), pp. 764-74. |
| DOI: |
10.4269/ajtmh.2012.11-0433 |
| Abstrakt: |
The present study analyzed the relationship between the genetic diversity of Plasmodium falciparum and parasitologic/entomologic indices in the Mount Cameroon region by using merozoite surface protein 1 as a genetic marker. Blood samples were collected from asymptomatic children from three altitude zones (high, intermediate, and low). Parasitologic and entomologic indices were determined by microscopy and landing catch mosquito collection/circumsporozoite protein-enzyme-linked immunosorbent assay, respectively. A total of 142 randomly selected P. falciparum-positive blood samples were genotyped by using a nested polymerase chain reaction-based technique. K-1 polymerase chain reaction products were also sequenced. As opposed to high altitude, the highest malaria prevalence (70.65%) and entomologic inoculation rate (2.43 infective/bites/night) were recorded at a low altitude site. Seven (18.91%), 22 (36.66%), and 19 (42.22%) samples from high, intermediate, and low altitudes, respectively, contained multiclonal infections. A new K-1 polymorphism was identified. This study shows a positive non-linear association between low/intermediate altitude (high malaria transmission) and an increase in P. falciparum merozoite surface protein 1 block 2 polymorphisms. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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