In vitro evaluation of Ficoll-enriched and genipin-stabilised collagen scaffolds.
| Autor: | Satyam A; Network of Excellence for Functional Biomaterials, National University of Ireland Galway, Galway, Ireland; Department of Mechanical & Biomedical Engineering, National University of Ireland Galway, Galway, Ireland., Subramanian GS, Raghunath M, Pandit A, Zeugolis DI |
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| Jazyk: | angličtina |
| Zdroj: | Journal of tissue engineering and regenerative medicine [J Tissue Eng Regen Med] 2014 Mar; Vol. 8 (3), pp. 233-41. Date of Electronic Publication: 2012 May 02. |
| DOI: | 10.1002/term.1522 |
| Abstrakt: | Polysaccharides are frequently incorporated into scaffolds for tissue engineering applications to improve mechanical and biological properties. We evaluated the influence of a Ficoll® scaffold on collagen films, a scaffold that is extensively used for soft and hard tissue repair. To avoid cytotoxicity issues associated with chemical reagents, the influence of genipin, a naturally occurring crosslinking agent, was assessed. Ultra-structural level collagen films formed with and without Ficoll showed a fine fibrillar structure whereas genipin crosslinked films showed a coarse fibrillar and partially nodular structure. In contrast, glutaraldehyde crosslinked films lost their fibrillar pattern. Crosslinking significantly increased denaturation temperature (p < 0.001), stress (p < 0.0001) and force (p < 0.0001) at break. Collagen/Ficoll and collagen/Ficoll/genipin films showed the highest WI38 fibroblast attachment than any other scaffold (p < 0.003) and significantly greater WI38 fibroblast metabolic activity than other scaffolds (p < 0.001). By day 6. collagen/Ficoll/genipin films also induced higher and more aligned fibronectin matrix deposition than other scaffolds. Overall, this study indicates the suitability of collagen/Ficoll/genipin for tissue engineering applications. (Copyright © 2012 John Wiley & Sons, Ltd.) |
| Databáze: | MEDLINE |
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