Autor: |
Siemens N; Institute of Medical Microbiology, Virology and Hygiene, Rostock University Hospital, Rostock, Germany., Fiedler T, Normann J, Klein J, Münch R, Patenge N, Kreikemeyer B |
Jazyk: |
angličtina |
Zdroj: |
Journal of bacteriology [J Bacteriol] 2012 Jul; Vol. 194 (14), pp. 3618-26. Date of Electronic Publication: 2012 Apr 27. |
DOI: |
10.1128/JB.00227-12 |
Abstrakt: |
Streptococcus pyogenes (group A streptococcus [GAS]) is a highly virulent Gram-positive bacterium. For successful infection, GAS expresses many virulence factors, which are clustered together with transcriptional regulators in distinct genomic regions. Ralp3 is a central regulator of the ERES region. In this study, we investigated the role of Ralp3 in GAS M49 pathogenesis. The inactivation of Ralp3 resulted in reduced attachment to and internalization into human keratinocytes. The Δralp3 mutant failed to survive in human blood and serum, and the hyaluronic acid capsule was slightly decreased. In addition, the mutant showed a lower binding capacity to human plasminogen, and the SpeB activity was significantly decreased. Complementation of the Δralp3 mutant restored the wild-type phenotype. The transcriptome and quantitative reverse transcription-PCR analysis of the serotype M49 GAS strain and its isogenic Δralp3 mutant identified 16 genes as upregulated, and 43 genes were found to be downregulated. Among the downregulated genes, there were open reading frames encoding proteins involved in metabolism (e.g., both lac operons and the fru operon), genes encoding lantibiotics (e.g., the putative salivaricin operon), and ORFs encoding virulence factors (such as the whole Mga core regulon and further genes under Mga control). In summary, the ERES region regulator Ralp3 is an important serotype-specific transcriptional regulator for virulence and metabolic control. |
Databáze: |
MEDLINE |
Externí odkaz: |
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