DNA damage in peripheral blood mononuclear cells of patients undergoing anti-tuberculosis treatment.

Autor: de Oliveira LRC; Department of Tropical Diseases, Botucatu School of Medicine - UNESP, São Paulo State University, Brazil. Electronic address: larissaragozo@yahoo.com.br., Peresi E; Department of Tropical Diseases, Botucatu School of Medicine - UNESP, São Paulo State University, Brazil., Tavares FC; Department of Tropical Diseases, Botucatu School of Medicine - UNESP, São Paulo State University, Brazil., Corrêa CR; Department of Internal Medicine, Botucatu School of Medicine - UNESP, São Paulo State University, Brazil., Pierine DT; Department of Internal Medicine, Botucatu School of Medicine - UNESP, São Paulo State University, Brazil., Calvi SA; Department of Tropical Diseases, Botucatu School of Medicine - UNESP, São Paulo State University, Brazil.
Jazyk: angličtina
Zdroj: Mutation research [Mutat Res] 2012 Aug 30; Vol. 747 (1), pp. 82-85. Date of Electronic Publication: 2012 Apr 15.
DOI: 10.1016/j.mrgentox.2012.04.003
Abstrakt: Tuberculosis (TB), a chronic infectious disease, is a major cause of morbidity and mortality worldwide. Expression of iNOS and consequent production of NO during the inflammatory process is an important defense mechanism against TB bacteria. We have tested whether pulmonary TB patients undergoing anti-tuberculosis treatment present DNA damage, and whether this damage is related to oxidative stress, by evaluating total hydrophilic antioxidant capacity and iNOS expression. DNA damage in peripheral blood mononuclear cells from patients and healthy tuberculin test (PPD) positive controls was evaluated by single-cell gel electrophoresis (comet assay), and iNOS expression was measured by qPCR. We also evaluated total hydrophilic antioxidant capacity in plasma from patients and controls. Compared to controls, pulmonary TB patients under treatment presented increased DNA damage, which diminished during treatment. Also, the antioxidant capacity of these individuals was increased at the start of treatment, and reduced during treatment. TB patients showed lower iNOS expression, but expression tended to increase during treatment. Our results indicate that pulmonary TB patients under anti-TB treatment exhibit elevated DNA damage in peripheral blood mononuclear cells. This damage was not related to nitric oxide but may be due to other free radicals.
(Copyright © 2012 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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