Rhizobium leguminosarum exoB mutants are deficient in the synthesis of UDP-glucose 4'-epimerase.

Autor: Canter Cremers HC; Department of Plant Molecular Biology, Leiden University, The Netherlands., Batley M, Redmond JW, Eydems L, Breedveld MW, Zevehuizen LP, Pees E, Wijffelman CA, Lugtenberg BJ
Jazyk: angličtina
Zdroj: The Journal of biological chemistry [J Biol Chem] 1990 Dec 05; Vol. 265 (34), pp. 21122-7.
Abstrakt: Rhizobium leguminosarum bv. viciae Exo- mutant strains RBL5523,exo7::Tn5,RBL5523,exo8::Tn5 and RBL5523,exo52::Tn5 are affected in nodulation and in the syntheses of lipopolysaccharide, capsular polysaccharide, and exocellular polysaccharide. These mutants were complemented for nodulation and for the syntheses of these polysaccharides by plasmid pMP2603. The gene in which these mutants are defective is functionally homologous to the exoB gene of Rhizobium meliloti. The repeating unit of the residual amounts of EPS still made by the exoB mutants of R. leguminosarum bv. viciae lacks galactose and the substituents attached to it. The R. leguminosarum bv. viciae and R. meliloti exoB mutants fail to synthesize active UDP-glucose 4'-epimerase.
Databáze: MEDLINE