Improvac does not modify the expression and activities of the major drug metabolizing enzymes cytochrome P450 3A and 2C in pigs.

Autor: Tomankova J; University of Veterinary and Pharmaceutical Science Brno, Faculty of Hygiene and Ecology, Department of Meat Hygiene and Technology, Brno, Czech Republic. tomankova.janicka@seznam.cz, Rasmussen MK, Andersson K, Ekstrand B, Zamaratskaia G
Jazyk: angličtina
Zdroj: Vaccine [Vaccine] 2012 May 21; Vol. 30 (24), pp. 3515-8. Date of Electronic Publication: 2012 Apr 04.
DOI: 10.1016/j.vaccine.2012.03.072
Abstrakt: In the present study, we investigated hepatic mRNA expression and activities of CYP3A and 2C in entire, surgically castrated and pigs vaccinated with Improvac. Additionally, we examined the mRNA expression of the two nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR), known to regulate CYP3A and 2C mRNA expression, respectively. Activities of CYP3A and 2C were estimated as a rate of 7-benzyloxy-4-trifluoromethylcoumarin and 7-benzyloxyquinoline metabolism (CYP3A) and tolbutamide metabolism (CYP2C). We found no effect of Improvac treatment or surgical castration on either CYP3A or 2C activities. Similarly, the mRNA expressions of CYP3A29, 2C33 and PXR were not changed. CAR mRNA expression differed only between entire and surgically castrated male pigs (p=0.005), being greater in surgically castrated pigs. Our results indicated that neither CYP3A nor 2C are affected by Improvac.
(Copyright © 2012 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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