| Autor: |
Rowther FB; Brain Tumour UK Neuro-oncology Research Centre, Research Institute in Healthcare Sciences, School of Applied Sciences, University of Wolverhampton, Wolverhampton, United Kingdom. fbrowther@wlv.ac.uk, Kardooni H, Warr T |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of biomolecular techniques : JBT [J Biomol Tech] 2012 Apr; Vol. 23 (1), pp. 1-3. |
| DOI: |
10.7171/jbt.12-2301-004 |
| Abstrakt: |
We report a unique amplification technique that works efficiently and specifically over a temperature range, rather than at one specific temperature, throughout the amplification process. As bisulfite-modified DNA is one of the difficult to amplify templates, we used this technique to amplify regions of promoter-associated CpG island for 11 genes using this template. This technique amplified specific products for every gene without requiring any optimization. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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