Autor: |
Guo R; Institute of Chemical Biology, Imperial College London, London SW7 2AZ, United Kingdom., Miele M, Gardner EM, Fournier F, Kornau KM, Gould IR, Klug DR |
Jazyk: |
angličtina |
Zdroj: |
Faraday discussions [Faraday Discuss] 2011; Vol. 150, pp. 161-74; discussion 257-92. |
DOI: |
10.1039/c1fd00007a |
Abstrakt: |
The ability to detect molecular complexes and determine their geometries is crucial to our understanding of all biological phenomena, including protein structures and functions. We recently demonstrated that a novel 2DIR technique, EVV 2DIR spectroscopy, can be used for this purpose. In this paper, we evaluate the potential utility of the method for the analysis of protein composition, structure and function. In order to do this we apply computational tools to a group of selected biological systems, for which our calculated spectra all showed features that can in principle be detected with existing sensitivities. We also investigate the possibility of using our technique to detect and analyse hydrogen-bonded systems through a tyrosine-water model. |
Databáze: |
MEDLINE |
Externí odkaz: |
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