| Autor: |
Davila JC; Department of Pharmacology and Toxicology, College of Pharmacy, University of Texas, Austin 78712-1074., Hsieh GC, Acosta D, Davis PJ |
| Jazyk: |
angličtina |
| Zdroj: |
Toxicology letters [Toxicol Lett] 1990 Nov; Vol. 54 (1), pp. 23-31. |
| DOI: |
10.1016/0378-4274(90)90051-m |
| Abstrakt: |
Cunninghamella echinulata was used to assess the cytotoxicity of papaverine (papaver), at concentrations ranging from 1 x 10(-5) to 1 x 10(-3) M for 1-6 days. Leakage of lactate dehydrogenase (LDH) and alanine aminotransferase (ALT), and changes in alkaline phosphatase (AP) activity and lactate/pyruvate (L/P) ratios were used as indices of cytotoxicity. Leakage of LDH occurred at the 6th day with 1 x 10(-3) M; AP activity and L/P ratios decreased after 6 days treatment with 1 x 10(-4) M, after 1 day with 1 x 10(-3) M, and after 3 days with 1 x 10(-4) M, respectively. This study provides support for the use of fungal cell systems to evaluate the toxicity of drugs and chemicals. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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