Regulation of proliferation and differentiation of mouse tooth germ epithelial cells by distinct isoforms of p51/p63.

Autor: Matsuura T; Division of Advanced Prosthetic Dentistry, Tohoku University Graduate School of Dentistry, 4-1 Seiryomachi Aoba-ku, Sendai 980-8575, Japan., Kawata VK, Nagoshi H, Tomooka Y, Sasaki K, Ikawa S
Jazyk: angličtina
Zdroj: Archives of oral biology [Arch Oral Biol] 2012 Aug; Vol. 57 (8), pp. 1108-15. Date of Electronic Publication: 2012 Mar 20.
DOI: 10.1016/j.archoralbio.2012.02.023
Abstrakt: Objectives: p51/p63 gene, one of the p53 families, is specifically expressed in tooth germ epithelial cells and is essential for tooth development. This study aims to elucidate roles of p51/p63 in ameloblastic cell differentiation.
Materials and Methods: We determined expression pattern of each of p51/p63 isoforms by reverse transcriptase-polymerase chain reaction (RT-PCR) and western blotting using emtg (epithelium of molar tooth germ)-1, -2, -3, -4, and -5 cell lines established from a mandibular molar tooth germ of p53-deficient mice and SF2 cells which differentiates into ameloblasts upon exposure to NT4. Furthermore, we investigated the function of p51/p63 in these cells by Tet system, which enables inducible expression and knock down of the target genes of interest by exposing cells to doxycycline.
Results: The expression of ΔNp51B/ΔNp63α, an isoform without transactivation domain, was detected at high level in immature cells, while the expression of TAp51/TAp63 isoforms, isoforms of with the transactivation domain, was detected at high level in mature cells. Moreover, induction of TAp51A/TAp63γ expression led to down-regulation of ΔNp51B/ΔNp63α expression and cell proliferation. Interestingly, this also led to up-regulation of ameloblastin expression, a differentiation marker of amelogenesis.
Conclusions: The results suggested that p51/p63 might regulate the cell proliferation and differentiation of tooth germ epithelial cells.
(Copyright © 2012 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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