| Autor: |
Blanc AM; Sección Virología, Facultad de Ciencias, Universidad de la República, Montevideo 11600, Uruguay., Berois MB, Tomé LM, Epstein AL, Arbiza JR |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of veterinary science [J Vet Sci] 2012 Mar; Vol. 13 (1), pp. 59-65. |
| DOI: |
10.4142/jvs.2012.13.1.59 |
| Abstrakt: |
Herpes simplex virus type-1 (HSV-1) amplicon vectors are versatile and useful tools for transferring genes into cells that are capable of stimulating a specific immune response to their expressed antigens. In this work, two HSV-1-derived amplicon vectors were generated. One of these expressed the full-length glycoprotein D (gD) of bovine herpesvirus 1 while the second expressed the truncated form of gD (gDtr) which lacked the trans-membrane region. After evaluating gD expression in the infected cells, the ability of both vectors to induce a specific gD immune response was tested in BALB/c mice that were intramuscularly immunized. Specific serum antibody responses were detected in mice inoculated with both vectors, and the response against truncated gD was higher than the response against full-length gD. These results reinforce previous findings that HSV-1 amplicon vectors can potentially deliver antigens to animals and highlight the prospective use of these vectors for treating infectious bovine rhinotracheitis disease. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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