Human respiratory epithelial cells from nasal turbinate expressed stem cell genes even after serial passaging.

Autor: Ruszymah BH; Tissue Engineering Centre, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Kuala Lumpur, Malaysia. ruszyidrus@gmail.com, Izham BA, Heikal MY, Khor SF, Fauzi MB, Aminuddin BS
Jazyk: angličtina
Zdroj: The Medical journal of Malaysia [Med J Malaysia] 2011 Dec; Vol. 66 (5), pp. 440-2.
Abstrakt: Current development in the field of tissue engineering led to the idea of repairing and regenerating the respiratory airway through in vitro reconstruction using autologous respiratory epithelial (RE). To ensure the capability of proliferation, the stem cell property of RE cells from the nasal turbinate should be evaluated. Respiratory epithelial cells from six human nasal turbinates were harvested and cultured in vitro. The gene expression of FZD-9 and BST-1 were expressed in passage 2 (P2) and passage 4 (P4). The levels of expression were not significant between both passages. The RE cells exhibit the stem cell properties, which remains even after serial passaging.
Databáze: MEDLINE