| Autor: |
Jayme DW; GIBCO-BRL, Life Technologies, Inc., 3175 Staley Road, 14072, Grand Island, NY, USA., Kubiak JM, Battistoni TA, Cady DJ |
| Jazyk: |
angličtina |
| Zdroj: |
Cytotechnology [Cytotechnology] 1996 Jan; Vol. 22 (1-3), pp. 255-61. |
| DOI: |
10.1007/BF00353946 |
| Abstrakt: |
We designed an Integrated Media Preparation System (IMPS) for continuous, on-line preparation of cell culture media and delivery to intermediate storage vessels or directly to a bioreactor. Key components of the IMPS include: a high precision, continuous fluid mixing device; formulation-specific liquid medium concentrates; validated process controls and membrane filtration; and automated dispensing into large volume flexible plastic containers. The IMPS system is designed to produce sterile, single-strength liquid medium from common raw materials at a delivery rate of 1000-3000 liters per hour and will manufacture homogenous batches from several thousand liters to over 60,000 liters. Fortified nutrient media prepared from multi-component 50X concentrates have been demonstrated to accelerate bioreactor seed chains, increase product yield, and reduce the overall manufacturing cost of nutrient medium. A productivity matrix will analyze the fully-loaded costs and contrast alternative methods for media preparation against projected biological yield. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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