Evaluation of the matrix effect of thermophilic anaerobic digestion on inactivation of infectious laryngotracheitis virus using real-time PCR and viral cell culture.

Autor: Gao T; Highmark Renewables Research LP, AFDP Room-1-037, 6004-118 Street, Edmonton, Alberta, Canada T6G 2E1. tj@highmark.ca, Bowlby E, Tong Y, Wu JT, Wong L, Tower RJ, Pang X, Li X
Jazyk: angličtina
Zdroj: Bioresource technology [Bioresour Technol] 2012 Apr; Vol. 110, pp. 692-6. Date of Electronic Publication: 2012 Feb 06.
DOI: 10.1016/j.biortech.2012.01.166
Abstrakt: The matrix effect of the thermophilic anaerobic digestion (TAD) process on inactivation of infectious laryngotracheitis virus (ILTV) was evaluated. Viral cell culture and real-time PCR were used for assessing removal of the viral infectivity and degradation of viral DNA, respectively. Results showed that the TAD-derived matrix alone can inactivate the virus and destroy the nucleic acid helix core of ILTV in a time-and- dose-dependent manner. No cytopathogenic effect (CPE) was observed in the cells exposed to ILTV pre-treated with TAD matrix for 1.5h in experiment 1 and for 16h in experiment 2. There was a significant statistical difference between TAD matrix treated and non-treated cultures (p<0.001, Chi-test). Amplifiable ILT viral DNA was reduced 2.27 log by 1.5h-treatment and was not present by 16h-treatment with TAD matrix, indicating complete viral DNA fragmentation. The TAD process is an environmentally friendly way for disposing of poultry biowaste and carcasses.
(Copyright © 2012 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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