The effect of systemic corticosteroid therapy on the expression of toll-like receptor 2 and toll-like receptor 4 in the cutaneous lesions of leprosy Type 1 reactions.

Autor: Walker SL; Department of Clinical Research, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK. drstevewalker@hotmail.com, Roberts CH, Atkinson SE, Khadge S, Macdonald M, Neupane KD, Ranjit C, Sapkota BR, Dhakal S, Hawksworth RA, Mahat K, Ruchal S, Hamal S, Hagge DA, Lockwood DN
Jazyk: angličtina
Zdroj: The British journal of dermatology [Br J Dermatol] 2012 Jul; Vol. 167 (1), pp. 29-35. Date of Electronic Publication: 2012 Jun 01.
DOI: 10.1111/j.1365-2133.2012.10891.x
Abstrakt: Background: Leprosy is complicated by immunological reactions which can occur before, during and after successful completion of multidrug therapy. Genetic studies have suggested that polymorphisms in toll-like receptors (TLRs) may affect the susceptibility of an individual with leprosy to developing Type 1 reactions.
Objectives: To examine the gene and protein expression of TLRs in the cutaneous lesions of leprosy Type 1 reactions at the onset of reaction and during systemic corticosteroid therapy.
Methods: Patients who were being treated for leprosy type 1 reactions with corticosteroids as part of a randomized controlled trial of corticosteroid treatment had skin biopsies performed before, during and at the end of treatment. The gene and protein expression of TLR2 and TLR4 were measured.
Results: We have demonstrated that the gene hARP-P0 is a suitable control gene for TLR gene expression studies in this population. The gene and protein expression of TLR2 and TLR4 were both reduced significantly during corticosteroid treatment.
Conclusions: This is the first study to examine the expression of TLR2 and TLR4 in vivo in individuals experiencing leprosy Type 1 reactions. The data support the possibility of an important role for TLR2 and TLR4 in the pathogenesis of this important complication of leprosy.
(© 2012 The Authors. BJD © 2012 British Association of Dermatologists.)
Databáze: MEDLINE
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