| Autor: |
Strasser SD; Biomedical Engineering Department, Northwestern University, Evanston, Illinois 60208, USA., Shekhawat G, Rogers JD, Dravid VP, Taflove A, Backman V |
| Jazyk: |
angličtina |
| Zdroj: |
Optics letters [Opt Lett] 2012 Feb 15; Vol. 37 (4), pp. 506-8. |
| DOI: |
10.1364/OL.37.000506 |
| Abstrakt: |
Quantification of intracellular nanoscale macromolecular density distribution is a fundamental aspect to understanding cellular processes. We report a near-field penetrating optical microscopy (NPOM) technique to directly probe the internal nanoscale macromolecular density of biological cells through quantification of intracellular refractive index (RI). NPOM inserts a tapered optical fiber probe to successive depths into an illuminated sample. A 50 nm diameter probe tip collects signal that exhibits a linear relationship with the sample RI at a spatial resolution of approximately 50 nm for biologically relevant measurements, one order of magnitude finer than the Abbe diffraction limit. Live and fixed cell data illustrate the mechanical ability of a 50 nm probe to penetrate biological samples. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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