| Autor: |
de Corato A; Institute of Pharmacology, Catholic University of Sacred Heart, 00168 Rome, Italy., Capuano A, Currò D, Tringali G, Navarra P, Dello Russo C |
| Jazyk: |
angličtina |
| Zdroj: |
Neuron glia biology [Neuron Glia Biol] 2011 May; Vol. 7 (2-4), pp. 109-16. Date of Electronic Publication: 2012 Feb 10. |
| DOI: |
10.1017/S1740925X11000172 |
| Abstrakt: |
In the present paper, we have further developed an in vitro model to study neuronal-glial interaction at trigeminal level by characterizing the effects of conditioned medium (CM) collected from activated primary cultures of satellite glial cells (SGCs) on calcitonin gene-related peptide (CGRP) release from rat trigeminal neurons. Moreover, we investigated whether such release is inhibited by a clinically relevant anti-migraine drug, sumatriptan. CM effects were tested on trigeminal neuronal cultures in different conditions of activation and at different time points. Long-term exposures of trigeminal neurons to CM increased directly neuronal CGRP release, which was further enhanced by the exposure to capsaicin. In this framework, the anti-migraine drug sumatriptan was able to inhibit the evoked CGRP release from naïve trigeminal neuron cultures, as well as from trigeminal cultures pre-exposed for 30 min to CM. On the contrary, sumatriptan failed to inhibit evoked CGRP release from trigeminal neurons after prolonged (4 and 8 h) pre-exposures to CM. These findings were confirmed in co-culture experiments (neurons and SGCs), where activation of SGCs or a bradykinin priming were used. Our data demonstrate that SGCs activation could influence neuronal excitability, and that this event affects the neuronal responses to triptans. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
