OMIP-006: phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry.

Autor: Murdoch DM; Division of Pulmonary and Critical Care Medicine, Duke University Medical Center, Durham, North Carolina, USA. david.murdoch@duke.edu, Staats JS, Weinhold KJ
Jazyk: angličtina
Zdroj: Cytometry. Part A : the journal of the International Society for Analytical Cytology [Cytometry A] 2012 Apr; Vol. 81 (4), pp. 281-3. Date of Electronic Publication: 2012 Feb 08.
DOI: 10.1002/cyto.a.22024
Abstrakt: This panel was optimized for the enumeration and phenotypic characterization of T regulatory cells (Tregs) within the CD4⁺ T-cell pool using human peripheral blood mononuclear cells (PBMC) using intranuclear and intracellular staining methods. The panel was optimized for HIV⁺ clinical trial specimens through the use of HIV-infected and normal donor PBMC. Because the panel is to be used in the context of testing cryopreserved PBMC obtained from multiple sites participating in clinical trials, it was essential to develop an assay that performed well using cryopreserved PBMC. Other tissue types have not been tested.
(Copyright © 2012 International Society for Advancement of Cytometry.)
Databáze: MEDLINE
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