| Autor: |
Edjème-Aké A; Laboratoire de biochimie, UFR sciences pharmaceutiques et biologiques, Université de Cocody, Abidjan, Côte d'Ivoire. angelake@aviso.ci, Garnotel R, Vallé-Polneau S, Ahiboh H, Hauhouot-Attoungbré ML, Monnet D, Gillery P |
| Jazyk: |
francouzština |
| Zdroj: |
Annales de biologie clinique [Ann Biol Clin (Paris)] 2012 Jan-Feb; Vol. 70 (1), pp. 13-7. |
| DOI: |
10.1684/abc.2011.0651 |
| Abstrakt: |
This study reports the comparison between two methods (chemiluminescence and enzymatic colorimetry) for revelation of apolipoprotein(a) [apo(a)] isoforms by immunoblotting in 102 Ivorian healthy subjects. Apo(a) isoform sizes were determined by sodium dodecyl sulfate-agarose-polyacrylamide gel electrophoresis (SDS-PAGE) followed by immunoblotting using enzymatic colorimetry or chemiluminescence. Within-run precision was comprised between 4.9% and 9.2% for colorimetry and between 2.9% and 4.6% for chemiluminescence. Both methods have detected apo(a) isoforms in all patients, even when lipoprotein(a) concentrations were under detection limit (0.02 g/L). The two methods were significantly correlated (r = 0.96 to 0.98, p<0.0001). Even though the chemiluminescence method exhibited better performances than the colorimetric method, both techniques could be used indifferently. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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