| Autor: |
Montecucchi PC; Sclavo SpA, DE.BI, Cassina dé Pecchi, Italy., Caldarini R, Sansó M, Bertolaso AM, Rosso di San Secondo VE, Piccolo G, Sirchia G |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography [J Chromatogr] 1990 Jul 20; Vol. 512, pp. 139-47. |
| DOI: |
10.1016/s0021-9673(01)89480-2 |
| Abstrakt: |
Procedure is described for purifying low-molecular-weight factors with antigen-aspecific properties from a dialysate of human leukocyte extract. It includes gel chromatography on Sephadex G-25 and G-15, ion-exchange chromatography, reversed-phase high-performance liquid chromatography (HPLC) on a C18 hydrophobic column and gel permeation HPLC. The immunosuppressive factor (mol.wt. 800-1000) was purified to near homogeneity. It is probably of peptidic nature, although it is pronase resistant. The enhancer factor (mol.wt. 300-600) is eluted from chromatographic columns together with a hypoxanthine-like substance. Nevertheless, the biological activity cannot be attributed to the purine derivative. Identification of this amplifier activity is still lacking. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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