Emergence of herpes simplex virus-1 syncytial variants with altered virulence for mice after selection with a natural carrageenan.
| Autor: | Mateu CG; Departamento de Química Biológica, Laboratorio de Virologia, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina., Recalde MP, Artuso MC, Hermida G, Linero FN, Scolaro LA, Damonte EB, Pujol CA, Carlucci MJ |
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| Jazyk: | angličtina |
| Zdroj: | Sexually transmitted diseases [Sex Transm Dis] 2011 Jun; Vol. 38 (6), pp. 555-61. |
| Abstrakt: | Background: Antiviral therapy against herpes simplex virus based on sulfated polysaccharides, like carrageenans, represents a new alternative for genital herpes infections treatment and arises the concern about the appearance of resistant viral populations. Methods: We characterized the F strain of herpes simplex virus-1 passaged in the presence of a natural carrageenan isolated from the red seaweed Gigartina skottbergii in view of the virulence for mice of isolated viral clones. Results: Viral clones (syn14-1 and syn17-2) showed a syncytial phenotype and a mild resistance to carrageenan, heparin, acyclovir, and brivudine. Both clones were avirulent for BALB/c mice when inoculated intravaginally, whereas F strain produced high mortality. Attenuation correlated with low levels of TNF-[alpha], interleukin-6, and IFN-[gamma] in vaginal lavages although virus titers were similar to those obtained for F strain. On the contrary, they showed a marked virulence when inoculated intranasally leading to a generalized spreading of virus. Conclusions: Results confirm the hypothesis that selection of herpes simplex virus-1 with a carrageenan in vitro leads to the emergence of variants with a differential virulence when compared to the original virus. This finding should be addressed when an antiviral therapy against genital herpes infection employing a natural carrageenan is under consideration. |
| Databáze: | MEDLINE |
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