Isolation, characterization and thin-layer chromatography method development of clerosterol palmityl ester: a chemical marker for standardization of leaves of Clerodendrum phlomidis.
| Autor: | Mohan Maruga Raja MK; Department of Pharmacognosy and Phytochemistry, Karnataka Kaveri Educational and Cultural Society College of Pharmacy, Bangalore, Karnataka, India. mohanmarugaraja@gmail.com, Mishra SH |
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| Jazyk: | angličtina |
| Zdroj: | Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine [Zhong Xi Yi Jie He Xue Bao] 2012 Jan; Vol. 10 (1), pp. 109-13. |
| DOI: | 10.3736/jcim20120116 |
| Abstrakt: | Objective: Clerosterol palmityl ester (CPE) is a unique clerosterol derivative isolated and characterized from the leaves of Clerodendrum phlomidis. Considering the uniqueness of this compound, the present study was planned to use CPE as a specific chemical marker and develop a new validated thin-layer chromatography (TLC) method for standardisation of C. phlomidis. Methods: Separation and quantification of CPE were achieved by TLC using a mobile phase of petroleum ether (60 to 80 degrees centigrade) and ethyl acetate (95:5, volume ratio) (Rf 0.64) on precoated silica gel 60F(254) aluminium plates. Densitometric determination was carried out after derivatization with anisaldehyde sulphuric acid reagent in absorption mode at 527 nm. Results: The calibration curve was linear in the concentration range of 100 to 500 ng/spot. The method was validated for precision, repeatability and accuracy. The proposed method was found to be simple, specific, precise, accurate, rapid and cost-effective. Conclusion: This TLC procedure may be used effectively for quantitative determination of CPE, identification of the plant and standardization of this plant or its derived products. |
| Databáze: | MEDLINE |
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