A new morphometric technique for assessment of melanization in skin of guinea pigs.
| Autor: | Anbar TS; Dermatology Department, Al- Minya University, Al- Minya, Egypt. taganbar@yahoo.com, El-Ammawi TS, Barakat MT, Abdel-Rahman AT, Fawzy A |
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| Jazyk: | angličtina |
| Zdroj: | Photodermatology, photoimmunology & photomedicine [Photodermatol Photoimmunol Photomed] 2012 Feb; Vol. 28 (1), pp. 42-6. |
| DOI: | 10.1111/j.1600-0781.2011.00636.x |
| Abstrakt: | Background: The degree of melanization is an important finding in many skin disorders. An objective measurement of melanin density is needed to achieve an accurate evaluation. Objectives: The present work aims at translating the subjective increase of melanin particles after narrow band ultraviolet-B (NB-UVB) irradiation into objective numerical values ready for statistical analysis. Materials and Methods: This study had involved 18 guinea pigs that were exposed to biweekly sessions of NB-UVB radiation for 4 weeks to induce skin pigmentation. Two skin biopsies were obtained from each animal; the first before treatment and the second at the end of the study, using 5 mm punch and stained with hematoxylin and eosin and Masson-Fontana (MF) stains. Surface area of both the epidermis (ESA) and the melanin particles (MPSA) were measured in μm(2) using a software supplied with Olympus light microscope. The MPSA/ESA percentage was calculated for each biopsy. The results before and after NB-UVB exposure were compared and statistically analyzed. Results: In the MF-stained sections, the mean ± SD of the MPSA/ESA percentage were 0.24 μm(2) ± 0.09 and 6.21 μm(2) ± 2.45 at the start of the study and at its end, respectively, with a highly significant difference (P < 0.001). Conclusion: This technique offers a new methodology for an accurate numerical evaluation of epidermal melanization. (© 2012 John Wiley & Sons A/S.) |
| Databáze: | MEDLINE |
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