| Autor: |
El'bert LB, Terletskaia EN, Timofeev AV, Amosenko FA, Khapchaev IuKh, Mironova LL, Svitkin IuV, Vorovich MF, Lisitsyna EA |
| Jazyk: |
ruština |
| Zdroj: |
Voprosy virusologii [Vopr Virusol] 1990 May-Jun; Vol. 35 (3), pp. 219-21. |
| Abstrakt: |
According to the WHO requirements, the concentration of cellular DNA in vaccine preparations produced by pooling virus from continuous cell lines is limited to 100 ng/dose. In this study, different methods were used for purification of tick-borne encephalitis virus suspensions grown in continuous cultures of cell line 4647 from cellular DNA. Two approaches are proposed based on treatment with DNAse and promamin sulfate which allow one to reduce cellular DNA concentration in the virus preparation to the acceptable level. Prospects of their use in vaccine production are discussed. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
