Electrochemical potential of Microgramma vaccinifolia rhizome lectin.

Autor: Santana GM; Departamento de Bioquímica, CCB, Universidade Federal de Pernambuco, Avenida Professor Moraes Rego s/n, 50670-420, Cidade Universitária, Recife, PE, Brazil., Albuquerque LP, Napoleão TH, Souza SR, Coelho LC, Paiva PM
Jazyk: angličtina
Zdroj: Bioelectrochemistry (Amsterdam, Netherlands) [Bioelectrochemistry] 2012 Jun; Vol. 85, pp. 56-60. Date of Electronic Publication: 2011 Dec 06.
DOI: 10.1016/j.bioelechem.2011.11.006
Abstrakt: This work reports the isolation of Microgramma vaccinifolia rhizome lectin (MvRL) and the determination of electrochemical potentials of MvRL in the presence of Ca²⁺, Mg²⁺ and human type O erythrocytes. MvRL showed the highest specific hemagglutinating activity with human type O erythrocytes and showed a single polypeptide band of 17 kDa on SDS-PAGE. MvRL hemagglutinating activity was neutralized after dialysis with EDTA, and addition of Ca²⁺ and Mg²⁺ restored the activity. Electrochemical potentials of MvRL in the presence of 100 mM Ca²⁺ (882 mV) and 60 mM Mg²⁺ (1051 mV) were higher (p<0.05) than in the presence of only 0.15 M NaCl (247 mV), indicating that the electrochemical system was sensitive to structural and physico-chemical changes promoted by these ions. MvRL potential did not change in the presence of type O erythrocytes. The electrochemical system was able to detect changes in electrochemical potentials of MvRL promoted by Ca²⁺ and Mg²⁺, even in a complex environment (human serum supplemented with 40 and 60mM of these ions). The study reveals that the stimulatory effect of Ca²⁺ and Mg²⁺ on hemagglutinating activity may be linked to conformational change and/or alterations in surface charge distribution of MvRL.
(Copyright © 2011 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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