Preparation of highly pure zeaxanthin particles from sea water-cultivated microalgae using supercritical anti-solvent recrystallization.

Autor: Chen CR; Department of Chemical Engineering, National Chung Hsing University, No. 250, Kuo-Kuang Road, Taichung 40227, Taiwan, ROC., Hong SE, Wang YC, Hsu SL, Hsiang D, Chang CM
Jazyk: angličtina
Zdroj: Bioresource technology [Bioresour Technol] 2012 Jan; Vol. 104, pp. 828-31. Date of Electronic Publication: 2011 Nov 30.
DOI: 10.1016/j.biortech.2011.11.083
Abstrakt: Xanthophylls, including zeaxanthin, are considered dietary supplements with a potentially positive impact on age-related macular degeneration. Using pilot-scale column fractionation coupled with supercritical anti-solvent (SAS) recrystallization, highly pure zeaxanthin particulates were prepared from ultrasonic extracts of the microalgae, Nannochloropsis oculata, grown in sea water. Column partition chromatography increased the concentration of zeaxanthin from 36.2 mg/g of the ultrasonic extracts to 425.6 mg/g of the collected column fractions. A response surface methodology was systematically designed for the SAS process by changing feed concentration, CO(2) flow rate and anti-solvent pressure. Zeaxanthin-rich particles with a purity of 84.2% and a recovery of 85.3% were produced using supercritical anti-solvent recrystallization from the column eluate at a feed concentration of 1.5 mg/mL, CO(2) flow rate of 48.6 g/min and pressure of 135 bar.
(Copyright © 2011 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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