Repeated administration of propofol upregulated the expression of c-Fos and cleaved-caspase-3 proteins in the developing mouse brain.
| Autor: | Cui Y; School of Pharmacy, Xuzhou Medical College, Xuzhou, Jiangsu, 221002, China., Ling-Shan G, Yi L, Xing-Qi W, Xue-Mei Z, Xiao-Xing Y |
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| Jazyk: | angličtina |
| Zdroj: | Indian journal of pharmacology [Indian J Pharmacol] 2011 Nov; Vol. 43 (6), pp. 648-51. |
| DOI: | 10.4103/0253-7613.89819 |
| Abstrakt: | Objectives and Aim: This study was designed to analyze the relationship between the expression of c-Fos protein and apoptosis in the hippocampus following propofol administration in infant mice. There are reports that certain drugs, including the general anesthetics applied in pediatrics and obstetrics, could block N-methyl-D-aspartate glutamate receptors and activate γ-aminobutyric acid type A receptors. Furthermore, some anesthetics could trigger neuroapoptosis and the expression of c-Fos in the developing rodent brain. Propofol is a general anesthetic increasingly used in pediatrics and obstetrics, and is reported to be able to interact with both γ-aminobutyric acid type A and N-methyl-D-aspartate glutamate receptors. No adequate evaluations have been available as to whether the dosage of propofol to maintain anaesthesia could trigger the expression of c-Fos and apoptosis. Materials and Methods: Intraperitoneal injections of propofol (50, 100 and 150 mg/kg) or vehicle were administered every 90 minutes (4 times) in infant mice (5-7 days old). 30 minutes after the final administration, the protein expressions of c-Fos and cleaved-caspase-3 in the hippocampus were determined by immunohistochemistry and Western blotting. Results: It was demonstrated that the expressions of cleaved-caspase-3 and c-Fos were upregulated in the hippocampal CA3 region in this study. Conclusions: The upregulated c-Fos expression induced by repeated injections of propofol might evoke neuroapoptosis. |
| Databáze: | MEDLINE |
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