Autor: |
Araújo FD; Chemistry Institute, University of Campinas, Campinas, SP, Brazil., Esper LM, Kuaye AY, Sircili MP, Marsaioli AJ |
Jazyk: |
angličtina |
Zdroj: |
Journal of agricultural and food chemistry [J Agric Food Chem] 2012 Jan 18; Vol. 60 (2), pp. 585-92. Date of Electronic Publication: 2012 Jan 04. |
DOI: |
10.1021/jf203846f |
Abstrakt: |
A chemical study of acyl-homoserine lactones (acyl-HSLs) produced by Enterobacter sakazakii resulted in the identification of three molecules: (S)-N-heptanoyl-HSL, (S)-N-dodecanoyl-HSL and (S)-N-tetradecanoyl-HSL. Mixed cultures of E. sakazakii and Bacillus cereus depleted E. sakazakii acyl-HSLs, suggesting acyl-HSL degradation by B. cereus hydrolases (hydrolysis of the lactone or amide moiety). The expression of B. cereus acyl-HSL lactonase and acyl-homoserine acylase was confirmed by monitoring the biotransformation of (S)-N-dodecanoyl-HSL into (S)-N-dodecanoyl-homoserine, dodecanoic acid and homoserine in the presence of B. cereus whole cells, using electrospray-mass spectrometry (ESI-MS). |
Databáze: |
MEDLINE |
Externí odkaz: |
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