The incorporation of the A2 protein to produce novel Qβ virus-like particles using cell-free protein synthesis.

Autor: Smith MT; Dept. of Chemical Engineering, Brigham Young University, Provo, UT 84602, USA., Varner CT, Bush DB, Bundy BC
Jazyk: angličtina
Zdroj: Biotechnology progress [Biotechnol Prog] 2012 Mar-Apr; Vol. 28 (2), pp. 549-55. Date of Electronic Publication: 2011 Nov 28.
DOI: 10.1002/btpr.744
Abstrakt: Virus-like particles (VLPs) have been employed for a number of nanometric applications because they self-assemble, exhibit a high degree of symmetry, and can be genetically and chemically modified. However, high symmetry does not allow for a single unique modification site on the VLP. Here, we demonstrate the co-expression of the cytotoxic A2 protein and the coat protein of the bacteriophage Qβ to form a nearly monodispersed population of novel VLPs. Cell-free protein synthesis allows for direct access and optimization of protein-synthesis and VLP-assembly. The A2 is shown to be incorporated at high efficiency, approaching a theoretical maximum of one A2 per VLP. This work demonstrates de novo production of a novel VLP, which contains a unique site that has the potential for future nanometric engineering applications.
(Copyright © 2011 American Institute of Chemical Engineers (AIChE).)
Databáze: MEDLINE
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