Demonstration of hydrogenase electrode operation in a bioreactor.

Autor: Shastik ES; Institute of Basic Biological Problems, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia., Vokhmyanina DV, Zorin NA, Voronin OG, Karyakin AA, Tsygankov AA
Jazyk: angličtina
Zdroj: Enzyme and microbial technology [Enzyme Microb Technol] 2011 Oct 10; Vol. 49 (5), pp. 453-8. Date of Electronic Publication: 2011 Sep 07.
DOI: 10.1016/j.enzmictec.2011.08.007
Abstrakt: This work describes the first step towards combination of the bioreactor with a starch-degrading microbial consortium and hydrogenase electrode (HE) in one unit for electricity generation. For this purpose, the bioreactor for microbial fermentation was designed with a set of electrodes (pH-sensor, Ag|AgCl reference electrode, Pt-electrode, and HE) inside the bioreactor. Potentials of all electrodes and H(2) accumulation were monitored in the system under the precise pH control. Results obtained with the hydrogen-producing microbial consortium indicated that HE generates the potential equal to the H(2)|2H(+) equilibrium potential. Furthermore, HE was able to catalyze the current generation (200 μA) by consuming H(2) gas produced in the microbial consortium from starch. After 220 h of operation, HE retained at least 81% of the initial activity. Calculations of carbon balance indicated that fermentation products were similar in microbial cells without HE and with HE generating the current due to H(2) consumption.
(Copyright © 2011 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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