| Autor: |
Venkatesan V; National Centre for Laboratory Animal Science, National Institute of Nutrition, Jamai Osmania, Hyderabad, India, v.venkateshan@gmail.com., Chalsani M, Nawaz SS, Bhonde RR, Challa SS, Nappanveettil G |
| Jazyk: |
angličtina |
| Zdroj: |
Cytotechnology [Cytotechnology] 2012 Mar; Vol. 64 (2), pp. 139-44. Date of Electronic Publication: 2011 Nov 23. |
| DOI: |
10.1007/s10616-011-9409-y |
| Abstrakt: |
WNIN/Ob, a mutant rat strain, developed at the National Center for Laboratory Animal Sciences (NCLAS) facility of National Institute of Nutrition (NIN), is a new animal model to study the metabolic syndrome. These animals have 47% fat in their body and isolation of islets from these animals were compounded due to the formation of amorphous viscous and jelly like material which reduced the islet yield. However, islets isolated from WNIN adult (≥12 months) control rats gave a good islet recovery, under standard isolation procedures using collagenase digestion. In the present study we optimized culture conditions in WNIN/Ob rats to isolate islets with higher yield, and also established primary islet cell cultures from these mutant rats, retaining cellular integrity and functionality. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Full text from SpringerLink