[Experimental study on stem cells from goat deciduous teeth compounded with porous calcium phosphate cement for ectopic osteogenesis].

Autor:	Zhao W; Department of Stomatology, Shanghai Jiao Tong University, Shanghai, China. zhwdzw1@126.com, Hao YM, Zou DR, Zhang XL, Lu JY, Hua L, Cao CH
Jazyk:	čínština
Zdroj:	Shanghai kou qiang yi xue = Shanghai journal of stomatology [Shanghai Kou Qiang Yi Xue] 2011 Oct; Vol. 20 (5), pp. 464-9.
Abstrakt:	Purpose: To observe the biocompatibility and ectopic bone-like tissue formation of stem cells from goat deciduous teeth (SGDs) with porous calcium phosphate cement (pCPC). Methods: The expression of STRO-1 on SGDs was measured with flow cytometry (FCM); the 4th passage SGDs were cultured in induced-mineralization medium in vitro for 7 days. Combined with pCPC, the adhesion and growth of the compounds were observed with scanning electron microscopy (SEM); the ectopic bone-like tissue formation was observed 8 weeks after the compounds implanted subcutaneously into the nude mice. Results: On the third day of SGDs compounded with pCPC, SEM verified that the cells adhered closely and tightly with pCPC, protruded pseudopods and secreted matrix. 8 weeks after the compounds implanted in ectopic sites, HE staining confirmed the formation of bone-like tissue; Immunohistochemistry showed the strongly positive expression of OCN protein in the implanted materials. Conclusions: SGDs may differentiate into osteoblast and are potential to induce bone matrix formation; combined with pCPC, the compounds may generate bone-like tissue.
Databáze:	MEDLINE
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