BJcuL, a lectin purified from Bothrops jararacussu venom, induces apoptosis in human gastric carcinoma cells accompanied by inhibition of cell adhesion and actin cytoskeleton disassembly.

Autor: Nolte S; Group for Advanced Molecular Investigation, PPGCS/CCBS, PUCPR, 80215-901 Curitiba, Paraná, Brazil., de Castro Damasio D, Baréa AC, Gomes J, Magalhães A, Mello Zischler LF, Stuelp-Campelo PM, Elífio-Esposito SL, Roque-Barreira MC, Reis CA, Moreno-Amaral AN
Jazyk: angličtina
Zdroj: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2012 Jan; Vol. 59 (1), pp. 81-5. Date of Electronic Publication: 2011 Nov 04.
DOI: 10.1016/j.toxicon.2011.10.012
Abstrakt: We show that BJcuL, a lectin purified from Bothrops jararacussu venom, exerts cytotoxic effects to gastric carcinoma cells MKN45 and AGS. This effect was due to the direct interaction with specific glycans on the cells surface and was observed by cell viability decrease, disorganization of actin filaments and apoptosis. In addition, BJcuL was able to reduce tumor cell adhesion to matrigel, what was inhibited by specific carbohydrate or partially inhibited when cells were pre-incubated with matrigel. Our results suggest that BJcuL was able to promote apoptosis in both tumor cells lines and therefore has a prospect for potential use in cancer therapy.
(Copyright © 2011 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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