| Autor: |
Azab HA; Chemistry Department, Faculty of Science, Suez Canal University, Ismailia, Egypt. azab2@yahoo.com, Hussein BH, El-Falouji AI |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of fluorescence [J Fluoresc] 2012 Mar; Vol. 22 (2), pp. 639-49. Date of Electronic Publication: 2011 Nov 09. |
| DOI: |
10.1007/s10895-011-1000-6 |
| Abstrakt: |
Eu(III)-9-acridinecarboxylate (9-ACA) complex was synthesized and characterized by elemental analysis, conductivity measurement, IR spectroscopy, thermal analysis, mass spectroscopy, (1)H-NMR, fluorescence and ultraviolet spectra. The results indicated that the composition of this complex is [Eu(III)-(9-ACA)(2)(NCS)(C(2)H(5)OH)(2)] 2.5 H(2)O and the oxygen of the carbonyl group coordinated to Eu(III). The interaction between the complex with nucleotides guanosine 5'- monophosphate (5'-GMP), adenosine 5'-diphosphates (5'-ADP), inosine (5'-IMP) and CT-DNA was studied by fluorescence spectroscopy. The fluorescence intensity of Eu(III)-9-acridinecarboxylate complex was enhanced with the addition of CT-DNA. The effect of pH values on the fluorescence intensity of Eu(III) complex was investigated. Under experimental conditions, the linear range was 9-50 ng mL(-1) for calf thymus DNA (CT- DNA) and the corresponding detection limit was 5 ng mL(-1). The results showed that Eu(III)-(9-ACA)(2) complex binds to CT-DNA with stability constant of 2.41 × 10(4) M. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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